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KMID : 0364019960290080816
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Korean Journal of Thoracic and Cardiovascular Surgery
1996 Volume.29 No. 8 p.816 ~ p.821
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Histopathological Changes in Cold-Stored Dog Lungs to the Preservation Solutions
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김해균
이두연/백효채/배기만/조현민/이기범/박만실/김현
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Abstract
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Lung transplantation is the established treatment for the end stage lung disease and preservation of the organ is a major obstacle in performing lung transplantation. For solving this problem, we evaluated the histopathologic changes for various
preservation solutions.
Male mongrel dogs of similar size and weight(15~20kg) were used. The dog lungs were flushed with 4℃ normal saline(group 1:n=5); Modified Euro-Collins solution(group 2: n=5) and University of Wisconsin solution(group 3:n=6), 60ml/kg through a
catheter
placed in the main pulmonary artery after flushing of PGEI(20ng/kg). The lungs were preserved for 60 hours and measured dry and wet weights Histologic specimens were taken every 6 hours and fixed for light microscopic evaluation.
The edema ratio of the lungs peaked in 12 hours although there was no difference between the groups. Histologically, alveolar septal changes developed in one case(20%) after I hour preservation with normal saline. In case of the University of
Wisconsin
solution, the alveolar septal distortions and swellings were seen in l cases(20%) after 6 hours preservation compared with 3 cases(60%) after 6 hours preservation with Modified Euro-Collins solution. Changes of the pneumocytes were observed after
24
hours preservation in group l, after 48 hours preservation in group 2 and after 60 hours preservation in group3.
We conclude that University of Wisconsin solution might have a superior preservation effect compare to normal saline and Modified Euro-Collins solutions.
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KEYWORD
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